SDF-1? Gene-Activated Collagen Scaffold Restores Pro-Angiogenic Wound Healing Features in Human Diabetic Adipose-Derived Stem Cells
نویسندگان
چکیده
Non-healing diabetic foot ulcers (DFUs) can lead to leg amputation in patients. Autologous stem cell therapy holds some potential solve this problem; however, cells are relatively dysfunctional and restrictive their wound healing abilities. This study sought explore if a novel collagen–chondroitin sulfate (coll–CS) scaffold, functionalized with polyplex nanoparticles carrying the gene encoding for stromal-derived factor-1 alpha (SDF-1? gene-activated scaffold), enhance regenerative functionality of human adipose-derived (ADSCs). We assessed impact scaffold on ADSCs by comparing response against healthy cultured gene-free over two weeks. Overall, we found that could restore pro-angiogenic similar scaffold. Gene protein expression analysis revealed induced overexpression SDF-1? engaged receptor CXCR7, causing downstream ?-arrestin signaling, as effectively transfected ADSCs. The also exhibited pro-wound features characterized active matrix remodeling provisional fibronectin basement membrane collagen IV. controlled pro-healing disabling early developmental factors signaling while promoting tissue components. Conclusively, show overcome deficiencies associated ADSCs, paving way autologous therapies combined biomaterials treat DFUs.
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ژورنال
عنوان ژورنال: Biomedicines
سال: 2021
ISSN: ['2227-9059']
DOI: https://doi.org/10.3390/biomedicines9020160